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immunofluorescence staining anti rabbit igg  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology immunofluorescence staining anti rabbit igg
    Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape <t>immunofluorescence</t> image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.
    Immunofluorescence Staining Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+thrombin+r/pm37488908-169-90-84?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 89 article reviews
    immunofluorescence staining anti rabbit igg - by Bioz Stars, 2026-08
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    1) Product Images from "Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathway."

    Article Title: Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathway.

    Journal: Neural regeneration research

    doi: 10.4103/1673-5374.375345

    Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape immunofluorescence image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.
    Figure Legend Snippet: Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape immunofluorescence image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.

    Techniques Used: Expressing, Activation Assay, Immunofluorescence, Fluorescence

    Figure 7 ｜Argatroban inhibits astrogliosis at the acute phase of SCI. (A) Immunofluorescence image of Vimentin (green) and PAR1 (red) at 3 days post-injury. DAPI was used to label cell nuclei (blue). The expression of Vimentin and PAR1 in the argatroban group were reduced compared with the SCI group. Scale bar: 25 µm, 100 µm (enlarged images). (B, C) Quantification of fluorescence intensities of Vimentin and PAR1. (D) Diagram of the transverse spinal cord section. The box indicates the observation site in the spinal cord. Data are shown as mean ± SEM (n = 3). ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2- phenylindole; PAR1: protease-activated receptor 1; SCI: spinal cord injury.
    Figure Legend Snippet: Figure 7 ｜Argatroban inhibits astrogliosis at the acute phase of SCI. (A) Immunofluorescence image of Vimentin (green) and PAR1 (red) at 3 days post-injury. DAPI was used to label cell nuclei (blue). The expression of Vimentin and PAR1 in the argatroban group were reduced compared with the SCI group. Scale bar: 25 µm, 100 µm (enlarged images). (B, C) Quantification of fluorescence intensities of Vimentin and PAR1. (D) Diagram of the transverse spinal cord section. The box indicates the observation site in the spinal cord. Data are shown as mean ± SEM (n = 3). ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2- phenylindole; PAR1: protease-activated receptor 1; SCI: spinal cord injury.

    Techniques Used: Immunofluorescence, Expressing, Fluorescence



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    Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape <t>immunofluorescence</t> image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.
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    Image Search Results


    Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape immunofluorescence image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.

    Journal: Neural regeneration research

    Article Title: Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathway.

    doi: 10.4103/1673-5374.375345

    Figure Lengend Snippet: Figure 8 ｜Argatroban decreases GFAP expression and astrocyte activation for repairing SCI. (A) The whole-landscape immunofluorescence image of GFAP (green) at 6 weeks post-injury. DAPI was used to label cell nuclei (blue). Immunopositivity of GFAP in the argatroban group was reduced compared with the SCI group. A1, A2, and A3 represent the epicenter of spinal cord injury. A1’–A3’ represent the GFAP channel and A1’’– A3’’ represent the merged channel of GFAP and DAPI. DAPI was used to label nuclei (blue). Scale bars: 1 mm (A1–3), 25 µm (A1’–A3’ and A1’’–A3’’). (B) Quantification of fluorescence intensity of GFAP in (A). (C) Quantification of fluorescence intensity of GFAP in epicenter in A1, A2, and A3. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury.

    Article Snippet: Concentration Anti-thrombin Rabbit Abcam, Cambridge, MA, USA ab92621 AB_10711179 1:1000 (western blotting) Anti-PAR1 Rabbit Gene Tex, Irvine, CA, USA GTX64534 NA 1:1000 (western blotting) Anti-JAK2 Rabbit CST, Danvers, MA, USA 3230S NA 1:1000 (western blotting) Anti-STAT3 Mouse Abcam ab119352 AB_10901752 1:1000 (western blotting) Anti-pSTAT3 Rabbit Abcam ab76315 AB_1658549 1:1000 (western blotting) Anti-GAPDH Mouse Santa Cruz Biotechnology, Hercules, CA, USA sc-32233 AB_627679 1:500 (western blotting) Anti-Vimentin Rabbit Abcam ab92547 AB_10562134 1:200 (Immunofluorescence staining) Anti-GFAP Rabbit Bioss, Beijing, China bs-0199R NA 1:200 (Immunofluorescence staining) Anti-PAR1 Mouse Santa Cruz Biotechnology sc-13503 NA 1:200 (Immunofluorescence staining) Anti-rabbit IgG, HRP-linked antibody Goat CST 7074S NA 1:1000 (western blotting) Anti-mouse IgG, HRP-linked antibody Goat CST 7076S NA 1:1000 (western blotting) Fluorescent Alexa Fluor 488 goat anti-rabbit Goat Beyotime, Shanghai, China a0423 AB_2891323 1:200 (Immunofluorescence staining) Cy3-labeled goat anti-mouse IgG (H+L) Goat Beyotime a0521 NA 1:200 (Immunofluorescence staining) GADPH: Glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; HRP: horseradish peroxidase; JAK2: Janus kinase 2; NA: not applicable; PAR1: proteaseactivated receptor 1; pSTAT3: phosphor-signal transducer and activator of transcription 3; STAT3: signal transducer and activator of transcription 3.

    Techniques: Expressing, Activation Assay, Immunofluorescence, Fluorescence

    Figure 7 ｜Argatroban inhibits astrogliosis at the acute phase of SCI. (A) Immunofluorescence image of Vimentin (green) and PAR1 (red) at 3 days post-injury. DAPI was used to label cell nuclei (blue). The expression of Vimentin and PAR1 in the argatroban group were reduced compared with the SCI group. Scale bar: 25 µm, 100 µm (enlarged images). (B, C) Quantification of fluorescence intensities of Vimentin and PAR1. (D) Diagram of the transverse spinal cord section. The box indicates the observation site in the spinal cord. Data are shown as mean ± SEM (n = 3). ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2- phenylindole; PAR1: protease-activated receptor 1; SCI: spinal cord injury.

    Journal: Neural regeneration research

    Article Title: Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathway.

    doi: 10.4103/1673-5374.375345

    Figure Lengend Snippet: Figure 7 ｜Argatroban inhibits astrogliosis at the acute phase of SCI. (A) Immunofluorescence image of Vimentin (green) and PAR1 (red) at 3 days post-injury. DAPI was used to label cell nuclei (blue). The expression of Vimentin and PAR1 in the argatroban group were reduced compared with the SCI group. Scale bar: 25 µm, 100 µm (enlarged images). (B, C) Quantification of fluorescence intensities of Vimentin and PAR1. (D) Diagram of the transverse spinal cord section. The box indicates the observation site in the spinal cord. Data are shown as mean ± SEM (n = 3). ***P < 0.001, ****P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DAPI: 4′,6-Diamidino-2- phenylindole; PAR1: protease-activated receptor 1; SCI: spinal cord injury.

    Article Snippet: Concentration Anti-thrombin Rabbit Abcam, Cambridge, MA, USA ab92621 AB_10711179 1:1000 (western blotting) Anti-PAR1 Rabbit Gene Tex, Irvine, CA, USA GTX64534 NA 1:1000 (western blotting) Anti-JAK2 Rabbit CST, Danvers, MA, USA 3230S NA 1:1000 (western blotting) Anti-STAT3 Mouse Abcam ab119352 AB_10901752 1:1000 (western blotting) Anti-pSTAT3 Rabbit Abcam ab76315 AB_1658549 1:1000 (western blotting) Anti-GAPDH Mouse Santa Cruz Biotechnology, Hercules, CA, USA sc-32233 AB_627679 1:500 (western blotting) Anti-Vimentin Rabbit Abcam ab92547 AB_10562134 1:200 (Immunofluorescence staining) Anti-GFAP Rabbit Bioss, Beijing, China bs-0199R NA 1:200 (Immunofluorescence staining) Anti-PAR1 Mouse Santa Cruz Biotechnology sc-13503 NA 1:200 (Immunofluorescence staining) Anti-rabbit IgG, HRP-linked antibody Goat CST 7074S NA 1:1000 (western blotting) Anti-mouse IgG, HRP-linked antibody Goat CST 7076S NA 1:1000 (western blotting) Fluorescent Alexa Fluor 488 goat anti-rabbit Goat Beyotime, Shanghai, China a0423 AB_2891323 1:200 (Immunofluorescence staining) Cy3-labeled goat anti-mouse IgG (H+L) Goat Beyotime a0521 NA 1:200 (Immunofluorescence staining) GADPH: Glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; HRP: horseradish peroxidase; JAK2: Janus kinase 2; NA: not applicable; PAR1: proteaseactivated receptor 1; pSTAT3: phosphor-signal transducer and activator of transcription 3; STAT3: signal transducer and activator of transcription 3.

    Techniques: Immunofluorescence, Expressing, Fluorescence